Emerging Methods for Enhancing Pluripotent Stem Cell Expansion
نویسندگان
چکیده
منابع مشابه
Large-Scale Expansion of Human Embryonic and Induced Pluripotent Stem Cells for Cell Therapy Applications
Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...
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from OXPHOS to glycolysis [6]. The pluripotent marker OCT4 affects several target genes (e.g. ATP5D) associated with energy metabolism which are in favor of glycolysis [1]. Various new hPSC culture systems have been developed in recent years, including defined serum-free media (e.g., mTESR, StemPro, and E8) and synthetic substrates (e.g., synthemax) [7,8]. How the subtle differences in these cu...
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Large numbers of human pluripotent stem cells (hPSCs) are needed to meet the high demands of a range of biomedical applications, including cell replacement therapies and drug screening. Recent advances in media formulations and cell culture platforms have addressed many previous challenges that have hindered efficient expansion. Understanding and addressing the remaining challenges will further...
متن کاملPluripotent stem cell lines.
The derivation of human embryonic stem cells 10 years ago ignited an explosion of public interest in stem cells, yet this achievement depended on prior decades of research on mouse embryonic carcinoma cells and embryonic stem cells. In turn, the recent derivation of mouse and human induced pluripotent stem cells depended on the prior studies on mouse and human embryonic stem cells. Both human e...
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Glaucoma, the worldwide leading cause of irreversible blindness, is characterized by progressive degeneration of the optic nerve and loss of retinal ganglion cells. Research into glaucoma pathogenesis has been hampered by difficulties in isolating and culturing retinal ganglion cells in vitro. However, recent improvements in laboratory techniques have enabled the generation of a variety of matu...
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ژورنال
عنوان ژورنال: Frontiers in Cell and Developmental Biology
سال: 2020
ISSN: 2296-634X
DOI: 10.3389/fcell.2020.00070